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NucleRatio

IHC application for automated analysis of Nuclear Biomarkers in tissue sections stained with H & DAB compounds; suitabe for analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67 biomarkers.

Details

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Input:

  • Image of tissue section stained with DAB and Hematoxilin (H) compounds
  • Whole Slide Image (WSI)
  • Single Image in TIFF format

Processing

  • Pre-processing filters (Gaussian, Sharpen, others)
  • Background Removal
  • Segmentation by Color Deconvolution
  • Post Processing Options (opening, closing, smoothing, others)
  • Noise Removal Options
  • Post Processing Filters (size, shape)
  • Results correction by regression analysis

Results:

  • % Ratio DAB-stained area / Total Stained Area (DAB + H)
  • Nuclei count
  • Image overlays of stained tissue for visual validation

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