NucleRatio
IHC application for automated analysis of Nuclear Biomarkers in tissue sections stained with H & DAB compounds; suitabe for analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67 biomarkers.
Input:
- Image of tissue section stained with DAB and Hematoxilin (H) compounds
- Whole Slide Image (WSI)
- Single Image in TIFF format
Processing
- Pre-processing filters (Gaussian, Sharpen, others)
- Background Removal
- Segmentation by Color Deconvolution
- Post Processing Options (opening, closing, smoothing, others)
- Noise Removal Options
- Post Processing Filters (size, shape)
- Results correction by regression analysis
Results:
- % Ratio DAB-stained area / Total Stained Area (DAB + H)
- Nuclei count
- Image overlays of stained tissue for visual validation
